Genomic structure of the ME491/CD63 antigen gene and functional analysis of the 5′-flanking regulatory sequences

https://doi.org/10.1016/S0006-291X(05)81004-6Get rights and content

Summary

Genomic structure of the ME491/CD63 antigen gene and promoter activity of the 5′-flanking regulatory sequences were studied. The antigen gene consists of eight exons that span 4 kilobase-pairs. Primer extension analysis with RNA from cultured human cells identified three major transcription initiation sites. The 5′-flanking region of exon 1 has features characteristic to promoters of many house-keeping genes and growth-regulating genes. The region is highly GC rich and contains potential binding sites for transcription factors such as Sp1 and ETF, but not a TATA box. The 5′-flanking sequence exerted strong promoter activity when linked to a reporter gene. Deletion mutant analysis of the 5′-flanking sequence has strongly suggested that a potential, binding site for AP-1 plays an important role in positively regulating the gene expression.

References (32)

  • HildrethJ.E.K. et al.

    Blood

    (1991)
  • MetzelaarM.J. et al.

    J. Biol. Chem.

    (1991)
  • AzorsaD.O. et al.

    Blood

    (1991)
  • FujitaT. et al.

    Cell

    (1987)
  • SenapathyP. et al.

    Methods Enzymol.

    (1990)
  • ParkerR. et al.

    Cell

    (1987)
  • BirdA.P.

    Trends Genet.

    (1987)
  • KageyamaR. et al.

    J. Biol. Chem.

    (1988)
  • KageyamaR. et al.

    J. Biol. Chem.

    (1989)
  • AngelP. et al.

    Cell

    (1987)
  • AtkinsonB. et al.

    Hybridoma

    (1985)
  • HottaH. et al.

    Cancer Res.

    (1988)
  • MetzelaarM.J. et al.
  • HottaH. et al.

    Melanoma Res.

    (1991)
  • SzalaS. et al.
  • WrightM.D. et al.

    J. Biol. Chem.

    (1990)
  • Cited by (35)

    • Human uroplakin lb gene structure and promoter analysis

      2002, Biochimica et Biophysica Acta - Gene Structure and Expression
    • Differential Cooperation between Regulatory Sequences Required for Human CD53 Gene Expression

      2001, Journal of Biological Chemistry
      Citation Excerpt :

      This element must be a basic component of the transcription machinery of the humanCD53 gene. Sp1 elements are also important for expression of other tetraspan antigens, such as CD63 (52) and CD9 (33). Ets/Pu.1 sequences play a major role in CD53 gene expression.

    View all citing articles on Scopus
    View full text