Binding of apoA-IV-phospholipid complexes to plasma membranes of rat liver

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Summary

Rat apoA-IV complexes with dimyristoyl phosphatidylcholine (apoA-IVDMPC) have been prepared and their ability to bind to purified rat liver plasma membranes investigated. Binding equilibrium at 37°C was reached in 30 minutes. Saturation binding experiments and subsequent analysis of the results with Scatchard plots gave results consistent with the presence of a single saturable binding site. DMPC or POPC unilamellar vesicles could not compete with ApoA-IV-DMPC for binding; apoA-I-DMPC competed only partially. ApoE-poor HDL effectively competed with apoA-IV-DMPC. The fact that binding could be greatly reduced (> 70%) by preincubating the membrane with pronase (18 μg/ml), supports the conclusion that a membrane protein is involved in binding. Based on these results, we speculate that the rapid catabolism of apoA-IV in plasma may be mediated by a specific uptake mechanism in the liver. The implications of these results support the hypothesis that apoA-IV is involved in reverse cholesterol transport.

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