Biochemical and Biophysical Research Communications
Isolation of mouse and human cDNA clones encoding a protein expressed specifically in osteoblasts and brain tissues*
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Pleiotrophin: Activity and mechanism
2020, Advances in Clinical ChemistryCitation Excerpt :The fact that PTN knockout mice showed lower bone density and growth in weight-bearing bones demonstrates the importance of PTN to bone development. In fact, PTN's expression in osteoclasts played a crucial role in the protein's discovery [4]. It is now known that PTN is found in cell matrices that are precursors to bone formation [85].
Seeding decellularized nerve allografts with adipose-derived mesenchymal stromal cells: An in vitro analysis of the gene expression and growth factors produced
2019, Journal of Plastic, Reconstructive and Aesthetic SurgeryCitation Excerpt :The resulting cDNA libraries were analyzed by qRT-PCR (C1000 Touch Thermal cycler, Bio-Rad, Hercules, CA) using SYBR Green detection, and specific primers for the panel of genes are given in Table 1. All genes were chosen from the literature because of their importance in nerve regeneration.14,28–39 All MSC-only samples were analyzed in triplicate, and all MSC-seeded nerves were analyzed in groups of five.
The osteocyte
2019, Principles of Bone BiologyThe role of pleiotrophin in bone repair
2014, InjuryCitation Excerpt :The term OSF-1 was attributed to the factor in the early 90s, due to the fact that a cDNA clone coding for PTN was isolated from the murine osteoblastic cell line MC3T3-E1. The human counterpart was also found in cDNA libraries from the human osteosarcoma cell line MG-63 [6]. Heteronuclear NMR studies have not concluded to PTN's conformation but have shown that PTN contains two β-sheet domains connected by a flexible linker.
Pleiotrophin Regulates the Retention and Self-Renewal of Hematopoietic Stem Cells in the Bone Marrow Vascular Niche
2012, Cell ReportsCitation Excerpt :Interestingly, PTN+ ECs also express CXCL12 and lepR, which can be expressed by both sinusoidal ECs and perivascular reticular cells (Dar et al., 2005; Ding et al., 2012; Ikejima et al., 2004; Sugiyama et al., 2006). Of note, a prior study (Tezuka et al., 1990) suggested that calvarial bone osteoblasts express PTN, but we found little evidence that osterix+ bone lineage cells expressed PTN in the BM. Commensurate with our findings that PTN was highly expressed by BM sinusoidal ECs, PTN was highly concentrated in BM supernatants and in the conditioned media of primary BM sinusoidal ECs in culture, but was undetectable in the PB of WT mice.
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The nucleotide sequences reported in this paper will appear in the DDBJ, EMBL and GenBank Nucleotide Sequence Databases under the accession numbers D90225 and D90226.