Lipoprotein-apoprotein exchange in aqueous systems: Relevance to the occurrence of APOA-I and APOC proteins in a common particle

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Summary

Human plasma high density lipoproteins (HDL) were labeled in vitro with [125I]apoA-I. Chromatography of the [125I]HDL on Sephacryl S-200 revealed that a certain fraction of [125I]apoA-I readily dissociates from the intact particle over a wide range of HDL concentrations. The relatively constant value of the dissociated apoA-I concentration observed at various HDL concentrations suggests that a “critical monomer concentration” of apoA-I is in equilibrium with the parent lipoprotein. Addition of [131I]apoC proteins to HDL induces additional dissociation of oligomeric apoA-I with the concomitant incorporation of apoC into a new particle of about 460,000 daltons.

References (12)

  • AlaupovicP.

    Protides of the Biological Fluids

    (1972)
  • SchaeferE.J. et al.

    J. Lipid Res.

    (1978)
  • BursteinM. et al.

    Adv. Lipid Res.

    (1973)
  • BrownW.V. et al.

    J. Biol. Chem.

    (1969)
  • BilheimerD.W. et al.

    Biochim. Biophys. Acta

    (1972)
  • ShepherdJ. et al.

    J. Lipid Res.

    (1978)
There are more references available in the full text version of this article.

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