Role of prostaglandin endoperoxides in the serum thiobarbituric acid reaction

https://doi.org/10.1016/0003-9861(81)90091-6Get rights and content

Abstract

In the presence of heme and reduced glutathione, prostaglandin (PG) endoperoxides underwent rapid conversion to malondialdehyde and 12l-hydroxy-5,8,10-heptadecatrienoic acid. In addition, PG endoperoxides as well as lipid peroxides produced malondialdehyde to yield a red pigment during the thiobarbituric acid reaction with different efficiencies. The relative rates of the reaction were: 1,1,3,3-tetraethoxypropane, 100; PGG2, 55; PGH2, 32; and 15-hydroperoxyarachidonic acid, 6. The thiobarbituric acid reactive materials in rabbit serum decreased by 25–60%, after intravenous administration of aspirin (a cyclo-oxygenase inhibitor) and with a concomitant decline of serum PG levels. These results, taken together, suggested that serum thiobarbituric acid values, considered to be an indicator of lipid peroxide levels, were to a significant extent due to PG endoperoxides and their derivatives.

References (28)

  • I.D. Desai et al.

    J. Lipid Res

    (1963)
  • K. Yagi

    Biochem. Med

    (1976)
  • S. Ohki et al.

    J. Biol. Chem

    (1979)
  • T. Shimizu et al.

    J. Biol. Chem

    (1979)
  • M. Hamberg et al.

    Anal. Biochem

    (1968)
  • L. Levine et al.

    Prostaglandins

    (1973)
  • P. Wlodawer et al.

    FEBS Lett

    (1979)
  • K. Watanabe et al.

    Biochem. Biophys. Res. Commun

    (1979)
  • D.H. Nugteren et al.

    Biochim. Biophys. Acta

    (1973)
  • H. Ohkawa et al.

    J. Lipid Res

    (1978)
  • M. Hamberg et al.

    J. Biol. Chem

    (1971)
  • T. Miyamoto et al.

    J. Biol. Chem

    (1976)
  • A.L. Tappel
  • W.G. Niehaus et al.

    Eur. J. Biochem

    (1968)
  • Cited by (108)

    • Synergistic effect of Betulinic acid, Apigenin and Skimmianine (BASk) in high cholesterol diet rabbit: Involvement of CD36-TLR2 signaling pathway

      2021, Cytokine
      Citation Excerpt :

      The total cell viability was measured by comparing the absorbance of normal hPBMCs at 540 nm. Cyclooxygenase enzyme activity was estimated according to method described previously [29]. The reaction mixture comprised of enzyme source, Tris-HCl buffer with pH 8, 5 mM Glutathione, 5 mM Hemoglobin, arachidonic acid (200 μM).

    View all citing articles on Scopus
    View full text