Decreased thermal stability of collagens containing analogs of proline or lysine

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Abstract

Fibroblasts were incubated with analogs of proline or lysine and the thermal stability of procollagen molecules containing the analogs was investigated using pepsin digestion at different temperatures as an enzymatic probe of conformation. The procollagens containing either 4-cis-hydroxy-l-proline, 3,4-dehydroproline, or 4,5-trans-dehydrolysine were less stable than normal procollagen and these abnormal collagens were largely in a non-triplehelical conformation within the cells at 37 °C. These results support the idea that procollagen molecules which are not in a triple-helical conformation are not secreted at a normal rate. Procollagens containing both 4,5-trans-dehydrolysine and a proline analog were much less stable than molecules containing a single type of analog. This result suggests that simultaneous administration of both types of analogs may have a greater effect on collagen accumulation in whole-animal experiments than administration of a single analog.

References (23)

  • P.B. Ramaley et al.

    Fed. Eur. Biochem. Soc. Lett

    (1971)
  • P. Dehm et al.

    Biochim. Biophys. Acta

    (1971)
  • R.L. Margolis et al.

    Arch. Biochem. Biophys

    (1971)
  • T. Takeuchi et al.

    Biochim. Biophys. Acta

    (1969)
  • J. Rosenbloom et al.

    J. Biol. Chem

    (1970)
  • J. Rosenbloom et al.

    J. Biol. Chem

    (1971)
  • P.J. Christner et al.

    J. Biol. Chem

    (1971)
  • J. Uitto et al.

    Biochim. Biophys. Acta

    (1972)
  • M. Harsch et al.

    Fed. Eur. Biochem. Soc. Lett

    (1972)
  • S.A. Jimenez et al.

    Biochem. Biophys. Res. Commun

    (1973)
  • P. Dehm et al.

    Biochim. Biophys. Acta

    (1972)
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    This work was supported by the National Institutes of Health Research Grants AM-14439, AM-14526, and the National Institute of Dental Research U.S.P.H.S. Grant DE-02623.

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