Regular ArticleA Graphical Method for Determining the Number of Essential Sites in Enzymes with Multiple Binding Sites for a Ligand
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Novel glutaredoxin activity of the yeast prion protein ure2 reveals a native-like dimer within fibrils
2009, Journal of Biological ChemistryCitation Excerpt :These results strongly suggest that the catalytic unit in Ure2 fibrils contains at least two subunits that interact with each other during catalysis. To investigate this further, we used the graphical method developed by Wang and Srivastava (32) to determine the number of essential sites for substrate binding in one catalytic unit of the oligomeric enzyme (see “Experimental Procedures”). Fig. 8A shows plots of [GSH]p/v0 versus 1/[GSH] at different values of p.
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