Elsevier

Developmental Biology

Volume 123, Issue 1, September 1987, Pages 10-16
Developmental Biology

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Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes

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Abstract

In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. 3H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.

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This work was supported by NSF Grant PCM 82–16917 to J.V.R., NIH postdoctoral fellowship F32 A1 06745-03-CLN to K.S., and an EMBO Fellowship to N.B.

1

Present address: CNR Center of Cytopharmacology, Via Vanvitelli 32, 20121 Milan, Italy.

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