Changes in arginine phosphate levels during early development of sea-urchin embryos

doi:10.1016/0014-4827(74)90264-X

Experimental Cell Research

Volume 88, Issue 2, October 1974, Pages 422-424

https://doi.org/10.1016/0014-4827(74)90264-X Get rights and content

Abstract

Up to 60 sec after insemination, the content of arginine phosphate in sea urchin eggs remained constant at the same level as unfertilized eggs (0.76 μmole/10⁶ eggs), and then began to increase, reaching a plateau (1.38 μmoles/10⁶ eggs) at 180 sec after insemination, which was maintained until cleavage. During the first and the second cleavage, the content changed cyclically, falling abruptly by 15 to 25 % just before furrowing, and then returning to the initial level just after the onset of cleavage.

References (18)

D Epel
Exptl cell res
(1969)
PE Scholander et al.
Exptl cell res
(1958)
AA Infante et al.
Biochim biophys acta
(1965)
T Yanagisawa
Exptl cell res
(1967)
T Yanagisawa
Exptl cell res
(1968)
EL Chambers et al.
Exptl cell res
(1953)
T Nakazawa et al.
Exptl cell res
(1970)
RT Hinegardner et al.
Exptl cell res
(1964)
T Ohnishi et al.
Embryologia
(1963)

There are more references available in the full text version of this article.

Cited by (4)

Changes in triacylglycerol, diacylglycerol and free fatty acids after fertilization in developing toad embryos
1986, Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
The content and fatty-acid composition of triacylglycerols, diacylglycerols and free fatty acids were analyzed from the unfertilized oocyte stage to the gastrula stage in the toad Bufo arenarum Hensel. Fertilization triggered a 30% and a 40% decrease in triacylglycerol and diacylglycerol, respectively. In contrast, free fatty acid increased continuously from oocyte to gastrula stage with an accumulation of palmitate predominating. However, the observed increase in free fatty acid was too small to account for the decreases in both neutral glycerides. The decrease in triacylglycerol might be a reflection of the activation of lipolytic enzymes and the subsequent oxidation of fatty acids to meet the increased metabolic energy requirements brought on by fertilization. The diminished diacylglycerol content due to fertilization may be accounted for, at least in part, by the utilization of diacylglycerol in the synthesis of membrane phospholipids, inasmuch as their decrease occurred simultaneously with an increase in phosphatidic acid. When cell-free homogenates taken from the three stages of development (unfertilized, fertilized and gastrula) were incubated in Tris-Ringer buffer for 90 min, free fatty acid accumulated. Triacylglycerol and diacylglycerol did not change substantially during this incubation period. This fact indicates that the free fatty acid released during incubation was not derived from neutral glycerides, but probably from membrane phospholipids. The release of free fatty acid was significantly greater in samples from the fertilized oocyte stage. The results described in this paper suggest that the synthesis of membrane phospholipids, including an enhanced turnover of the acyl moiety, plays a significant role in the metabolic events activated by fertilization.
CHANGES IN ACTIVITIES OF CREATINE KINASE, ARGININE KINASE AND THEIR MULTIENZYME FORMS DURING EMBRYONIC AND LARVAL DEVELOPMENT OF SEA URCHINS
1978, Development, Growth & Differentiation
EFFECT OF DNP ON ACCELERATION OF THE CLEAVAGE FOLLOWING INSUFFICIENT PARTHENOGENETIC ACTIVATION IN THE SEA URCHIN EGG
1976, Development, Growth & Differentiation
CHANGES IN THE LEVEL OF ARGININE PHOSPHATE FOLLOWING FERTILIZATION OF ECHINODERM EGGS
1976, Development, Growth & Differentiation

^☆: This work was supported by a grant-in-aid for Fundamental Scientific Research from the Ministry of Education of Japan.

View full text

Experimental Cell Research

Preliminary noteChanges in arginine phosphate levels during early development of sea-urchin embryos☆

Abstract

Exptl cell res

Exptl cell res

Biochim biophys acta

Exptl cell res

Exptl cell res

Exptl cell res

Exptl cell res

Exptl cell res

Embryologia

Changes in triacylglycerol, diacylglycerol and free fatty acids after fertilization in developing toad embryos

CHANGES IN ACTIVITIES OF CREATINE KINASE, ARGININE KINASE AND THEIR MULTIENZYME FORMS DURING EMBRYONIC AND LARVAL DEVELOPMENT OF SEA URCHINS

EFFECT OF DNP ON ACCELERATION OF THE CLEAVAGE FOLLOWING INSUFFICIENT PARTHENOGENETIC ACTIVATION IN THE SEA URCHIN EGG

CHANGES IN THE LEVEL OF ARGININE PHOSPHATE FOLLOWING FERTILIZATION OF ECHINODERM EGGS

Preliminary note
Changes in arginine phosphate levels during early development of sea-urchin embryos☆