Original ArticlesDevelopment of a probe and PCR primers specific to the virulence plasmid of Salmonella enteritidis
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Digital polymerase chain reaction duplexing method in a single fluorescence channel
2023, Analytica Chimica ActaCitation Excerpt :In 2020, because of the COVID-19 pandemic, PCR became a standard method of choice for determining the presence of the SARS-CoV-2 virus. Three fundamental variants are currently available: standard (end-point detection) PCR [5], real-time or quantitative PCR (qPCR) [6], and digital PCR (dPCR) [7,8]. The development of dPCR has been enabled by microfluidic technology [9], in which the PCR master mix, including a DNA template, is compartmentalized into thousands or even millions of individual partitions, each containing either one or no copy of DNA, thus digitizing the template.
Designing a biochip following multiplex polymerase chain reaction for the detection of Salmonella serovars Typhimurium, Enteritidis, Infantis, Hadar, and Virchow in poultry products
2018, Journal of Food and Drug AnalysisCitation Excerpt :Over the past decades, polymerase chain reaction (PCR) has been frequently used for the detection of Salmonella and other foodborne pathogens [2,15,16]. A number of genus- and serovar-specific genes have been used for designing primers or probes specific for the detection of Salmonella serovars Enteritidis, Typhimurium, Typhi, Choleraesuis, Paratyphi, Hadar, and other pathogens [17–25]. For S. Infantis, based on fliB gene, specific PCR primers also have been designed [26].
Toward a better guard of coastal water safety—Microbial distribution in coastal water and their facile detection
2017, Marine Pollution BulletinCitation Excerpt :A β-glucuronidase operon, S-fimbrial operon, and cell surface-related genes were amplified. To probe the virulence genes carried on the plasmid of S. enteritidis, a 2-kb Pstl/Bgll fragment unique to this bacterium was utilized to design probes (Wood et al., 1994). In addition to chromosome-borne genes, virulence genes that are carried on a transportable vector can also be used as targets for tracking its transmission to similar hosts that are otherwise nonpathogenic.
Rapid identification of Salmonella enterica serovars, Typhimurium, Choleraesuis, Infantis, Hadar, Enteritidis, Dublin and Gallinarum, by multiplex PCR
2011, Journal of Microbiological MethodsCitation Excerpt :The second category consists of serovar-specific genes predicted by an approach in comparative genomics. These serovar-specific genes have been used to detect serovars Typhi, Typhimurium, Enteritidis, Choleraesuis and Paratyphi C (Charlton et al., 2005; Kim et al., 2006; Pan and Liu, 2002; Park et al., 2009; Shanmugasundaram et al., 2009; Wood et al., 1994; Woods et al., 2008). However, the specificity of these methods was not fully documented in these studies.
Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR)
1999, International Journal of Food Microbiology