Original Articles
Development of a probe and PCR primers specific to the virulence plasmid of Salmonella enteritidis

https://doi.org/10.1006/mcpr.1994.1068Get rights and content

Abstract

Restriction analysis of the Salmonella enteritidis virulence plasmid, followed by hybridisation with radiolabelled S. typhimurium and S. dublin plasmid, revealed a 2-kb PstIBglI fragment that was specific to S. enteritidis. Colony hybridisation experiments with this fragment detected 29 out of 31 S. enteritidis strains tested. S. blegdam, S. moscow and S. paratyphi C also hybridised with this fragment and a comparison of the plasmids from these serotoypes revealed striking similarities. A PstI/PvuII sub-clone of the 2-kb fragment was used to design primers for quantitative polymerase chain reaction (QPCR) detection of S. enteritidis from broth culture.

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