Two components in crystalline chlorophyll lipoprotein,☆☆

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Abstract

Crystalline chlorophyll lipoprotein (CLP) was obtained from isolated chloroplast material by a modification of Takashima's procedure. The CLP solution, chromatographed on filter paper with picoline-water (1:1) as a solvent, gave two bands, suggesting the presence of two components.

The two components were separated by column chromatography, using the pulp of filter paper as an adsorbent. Two zones, a blue-green zone above and a contiguous yellow-green one below, were differentiated after developing with 55 % picoline solution.

The blue-green and yellow-green pigments extracted from each zone were identified as belonging to the chlorophyll a and b series, by paper chromatography and by measuring their absorption spectra.

Crystals were obtained from the two solutions eluted from both zones with 55% picoline solution. The two kinds of crystals differ in their crystal forms (cf. Fig. 4).

Evidently CLP consists of two components, which contain separately chlorophylls a and b.

References (3)

  • S. Takashima

    Nature

    (1952)
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Cited by (1)

Contribution No. 33 from the Department of Biology, Faculty of Science, Kyushu University.

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The expense of this work was partly defrayed by a grant from the Science Research Fund of the Ministry of Education.

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