Preparation of lodinated cyclic GMP derivatives by a lactoperoxidase method

doi:10.1016/0003-2697(77)90256-1

Analytical Biochemistry

Volume 77, Issue 2, February 1977, Pages 429-435

https://doi.org/10.1016/0003-2697(77)90256-1 Get rights and content

Abstract

Preparation of ¹²⁵I-labeled succinyl cyclic GMP-tyrosine methyl ester (¹²⁵I-SCGMP-TME) was described. The method utilized lactoperoxidase and the glucose-glucose oxidase peroxide-generating system. ¹²⁵I-SCGMP-TME, purified by Sephadex G-10 gel filtration, was separated from cyclic GMP, SCGMP-TME, ¹²⁵I, and ¹²⁵I-labeled TME and retained extremely high immunoreactivity. A cyclic GMP radioimmunoassay, based on this iodinated material and cyclic GMP antiserum, is specific, sensitive, and reliable, permitting the determination of small quantities of cyclic GMP in rat tissue without need for chromatographic steps.

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¹: Present address: Third Department of Internal Medicine, Hiroshima University, School of Medicine, Kasumi-cho, Hiroshima, Japan.

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Preparation of lodinated cyclic GMP derivatives by a lactoperoxidase method

Abstract

J. Biol. Chem

Biochim. Biophys. Acta

J. Clin. Endocrinol

J. Clin. Endocrinol