Chemical degradation of 3H-labeled substance P in Tris buffer solution
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Cited by (10)
Considerations for Defining the Intravenous Administration Procedure for Nano-dose Sterile Drug Product in Clinical Studies
2022, Journal of Pharmaceutical SciencesCitation Excerpt :Therefore, the compatibility and recovery of the drug substance will be established prior to finalizing the clinical and commercial drug product, which ensures that an appropriate dosing kit is selected to deliver the potency, quality and safety of the product for human use. The loss of drug substance to the surfaces of dosing kits is more pronounced for low strength and/or low dose drug products.12 For example, Higa et al., have demonstrated that ultra-low dose substance P, an undecapeptide amide, significantly adsorbed onto untreated polypropylene and glass surfaces but the potency was effectively increased by coating the surfaces with bovine serum albumin (BSA).12
Transglutaminase-mediated conjugation and nitride-technetium-99m labelling of a bis(thiosemicarbazone) bifunctional chelator
2018, Journal of Inorganic BiochemistryCitation Excerpt :The fully optimized labelling reaction was then applied to the synthesis of 99mTc2. For this compound, the second step of the labelling procedure was performed in an Eppendorf Protein LoBind tube and in presence of γ-cyclodextrin (5 mg/mL), otherwise the SP-conjugated ligand and the final complex stick in the vial surface (data not shown) [57]. The RCY was about 72%; a radiochemically pure compound as showed in Scheme 1 D was achieved by RP-HPLC purification from the labelling mixture.
Sample preparation, high-performance liquid chromatographic separation and determination of substance P-related peptides
1995, Journal of Chromatography B: Biomedical Sciences and ApplicationsCharacterization of neuropeptides extracted from canine intestine
1993, NeuropeptidesSubstance P in Solution: Trans-to-Cis Configurational Changes of Penultimate Prolines Initiate Non-enzymatic Peptide Bond Cleavages
2019, Journal of the American Society for Mass Spectrometry