A novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles

doi:10.1016/0003-2697(91)90430-2

Analytical Biochemistry

Volume 198, Issue 2, 1 November 1991, Pages 308-311

https://doi.org/10.1016/0003-2697(91)90430-2 Get rights and content

Abstract

The paper describes a sensitive latex hybridization assay (LHA) method applied for indirect detection of biotinylated nucleic acid hybrids immobilized on a synthetic membrane. The biotinylated hybrids were visualized by means of latex particles containing the fluorescent dye pyronine G and coated with streptavidin; 1.6 and 0.3 pg of λ-phage DNA was detected by dot blot hybridizations on nylon membrane and polyethylenimine-cellophane, respectively. The assay sensitivity was increased by three orders of magnitude over that with fluorescently labeled probes due to incapsulation of the fluorescent dye in polymer particles. LHA is simple (single-stage detection procedure), fast, and more sensitive than any of the other nonradioactive hybridization methods.

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A novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles

Abstract

Mol. Cell. Probes

Anal. Biochem

FEBS Lett

Anal. Biochem

Anal. Biochem

Anal. Biochem

Science

Nucleic Acids Res