Elsevier

Analytical Biochemistry

Volume 88, Issue 2, 1 August 1978, Pages 682-688
Analytical Biochemistry

Degradation of tropoelastin by proteases

https://doi.org/10.1016/0003-2697(78)90473-6Get rights and content

Abstract

A simple assay is described which is capable of detecting single breaks in purified radioactively labeled tropoelastin after incubation with dilute solutions of proteases. Using this assay we show that tropoelastin is rapidly cleaved by a variety of proteases, including leukocyte and pancreatic clastase, at physiologic pH and ionic strength. The results suggest the possibility that degradation of tropoelastin or other related biosynthetic intermediates may play a role in the pathogenesis of emphysema.

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