Elsevier

Analytical Biochemistry

Volume 133, Issue 2, September 1983, Pages 336-343
Analytical Biochemistry

Inter-α-trypsin inhibitor (ITI): Use of new antisera for qualitative studies and discrete quantitation of ITI and its derivatives

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Abstract

Inter-α-trypsin inhibitor (ITI) is a human protease inhibitor characterized by its coexistence with several physiological derivatives displaying immunological cross-reactivity when analyzed in plasma with usual anti-ITI antisera. Taking advantage of the presence in urine of a particular ITI derivative, antisera with restricted specificity for either ITI or its derivatives could be prepared. Some applications of these new reagents are given, including qualitative studies and discrete quantitation—by electroimmunoassay—of ITI and ITI derivatives. The procedures herein described should prove useful for qualitative and quantitative analysis to investigators dealing with mixtures of antigenically related proteins.

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