Elsevier

Analytical Biochemistry

Volume 127, Issue 1, 15 November 1982, Pages 164-170
Analytical Biochemistry

Determination of nitrate reductase with laser-induced thermal-lensing colorimetry

https://doi.org/10.1016/0003-2697(82)90160-9Get rights and content

Abstract

Laser-induced thermal-lensing effect was applied to the measurement of nitrate reductase with Lowe's method. An Ar+ laser (514.5 nm) was used as the heat and probe sources. Signal processing for calibration was compared with respect to the steady-state method (with a personal computer system) and the transient method (with a transient memory-signal averager system). The least detectable amount of nitrate reductase is 5 × 10−7 unit/ml, which corresponds to 20 times that of the ordinary colorimetry. The dissolved nitrate reductase activity in the pond water was observed by using the present method.

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