Elsevier

Analytical Biochemistry

Volume 101, Issue 2, 15 January 1980, Pages 504-506
Analytical Biochemistry

Enzymatic determination of l-alanine with ω-amino acid:pyruvate aminotransferase

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Abstract

A simple and specific method with bacterial ω-amino acid:pyruvate aminotransferase and lactate dehydrogenase has been reported for the determination of l-alanine. This method involves a transamination of l-alanine with sulfoacetaldehyde to produce pyruvate and the spectrometric determination of this product with the aid of lactate dehydrogenase.

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