Bacterial adherence on replicas of sodium dodecyl sulfate-polyacrylamide gels☆
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Helicobacter pylori Adhesion to Carbohydrates
2006, Methods in EnzymologyCitation Excerpt :The in vitro tissue binding assay is valuable for initial characterization of microbial adherence to specific host cell lineages and receptors that the microbe exploits, and of variant strains, whether generated in the laboratory or recovered during the course of natural or experimental infection. Various overlay techniques on immobilized glycolipids or glycoproteins had been developed on the basis of bacterial overlay on either thin‐layer chromatography (Hansson et al., 1985; Karlsson and Stromberg, 1987) or nitrocellulose transfers of protein extracts separated by SDS‐PAGE (Prakobphol et al., 1987) to identify molecules carrying carbohydrate receptors for bacterial lectin‐like adhesins. Carbohydrate receptors are immobilized in these assays which facilitates detection of low‐affinity binding that depends on multivalent interactions between multiple adhesins on bacterial surfaces and clustered receptors on the solid‐phase substrate.
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1998, Veterinary MicrobiologyEvaluation of major parotid glycoproteins in patients with burning mouth syndrome
1997, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and EndodonticsIdentifying bacterial receptor proteins and quantifying strength of interactions they mediate
1995, Methods in EnzymologyDetection of glycoprotein receptors on blotting membranes by binding of live bacteria and amplification by growth
1995, Analytical Biochemistry
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This investigation was supported by USPHS Research Grant DE-07244 from the National Institute of Dental Research, National Institutes of Health, Bethesda, MD.