Structure of the carbohydrate chains of the proline-rich glycoprotein from human parotid saliva

https://doi.org/10.1016/0006-291X(82)91331-6Get rights and content

Abstract

The carbohydrate structure of a proline-rich glycoprotein from human parotid saliva is presented. Studies were performed on tritiated glycopeptides prepared by extensive pronase digestion of galactose oxidase/sodium borotritide treated glycoprotein. Structural characterization was carried out using a combination of periodate oxidation, methylation analysis, nitrous acid deamination, and glycosidase treatment. A triantennary asparagine-linked structure was found which contained peripheral Galβ1→4 (Fucα1→3) GlcNAc sequences.

References (15)

  • M.J. Levine et al.

    Arch. Oral Biol

    (1973)
  • M.J. Levine et al.

    Biochim. Biophys. Acta

    (1969)
  • H.C. Li et al.

    Arch. Oral Biol

    (1980)
  • M.J. Kessler et al.

    J. Biol. Chem

    (1979)
  • L. Warren

    J. Biol. Chem

    (1959)
  • O.P. Bahl et al.

    Biochem. Biophys. Res. Commun

    (1980)
  • M.J. Levine et al.

    Arch. Oral Biol

    (1973)
There are more references available in the full text version of this article.

Cited by (34)

  • Developments in diagnostic applications of saliva in human organ diseases

    2022, Medicine in Novel Technology and Devices
    Citation Excerpt :

    It is the fifth most commonly diagnosed cancer, affecting approximately one million people every year, and the third cause of cancer death [97]. To detect GC, biomarkers of saliva have been studied to focus on different target molecules, such as amino acids [98,99], proteins [38], and glycoproteins [100] or extracellular RNA [101]. Among them, the detection of target amino acids seems to be the method closest to point-in-time applications, because two types of sensors have been described.

  • Proline-rich salivary proteins have extended conformations

    2010, Biophysical Journal
    Citation Excerpt :

    Its N-glycosylation synthesis pathway mirrors that of typical mammalian cells up to the point where Man8GlcNAc2 N-glycosylated proteins exit the endoplasmic reticulum. These proteins are a model of human salivary N-glycosylated PRPs (42–44). For proteins that may be partly or fully unstructured, there are some well established programs (http://www.disprot.org) that predict the extent of folding and structural order of their conformations in solution, on the basis of the amino acid sequence (45).

  • Evaluation of major parotid glycoproteins in patients with burning mouth syndrome

    1997, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
  • Drug delivery using buccal-adhesive systems

    1993, Advanced Drug Delivery Reviews
View all citing articles on Scopus
View full text