Characterization of human interleukin-3 receptors on a multi-factor-dependent cell line

doi:10.1016/0006-291X(89)91553-2

Biochemical and Biophysical Research Communications

Volume 161, Issue 1, 30 May 1989, Pages 16-22

https://doi.org/10.1016/0006-291X(89)91553-2 Get rights and content

Abstract

Recombinant human interleukin-3 (hIL-3) was radioiodinated by Bolton-Hunter method with maintenance of biological activity. Using ¹²⁵I-hIL-3, hIL-3 receptors were characterized on a multi-factor-dependent cell line TF-1. Equilibrium binding studies revealed the existence of a single class of binding sites (667 ± 306 sites/cell) with a Kd of 173 ± 25 pM. Affinity labeling of TF-1 cells with ¹²⁵I-IL-3 yielded two bands of 150 kDa and 85 kDa, implying molecular weights of 135 kDa and 70 kDa for the hIL-3 receptors.

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Characterization of human interleukin-3 receptors on a multi-factor-dependent cell line

Abstract

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