Intermediates in flavoprotein catalyzed hydroxylations

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Abstract

The reaction of molecular oxygen with the complex of reduced p-hydroxybenzoate hydroxylase and 2,4-dihydroxybenzoate has been followed by rapid reaction techniques. During the reaction, which produces stoichiometric amounts of oxidized enzyme and the hydroxylated product, 2,3,4-trihydroxybenzoate, three spectroscopically distinguishable intermediates have been detected and characterized.

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