Flavin binding by bacterial luciferase: Affinity chromatography of bacterial luciferase

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Abstract

Immobilized FMN covalently attached to Sepharose-6B-hexanoate binds bacterial luciferase. Immobilized flavin is also effective in its reduced form as a substrate in the light emitting reaction catalyzed by luciferase.

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Predoctoral Fellow, National Institutes of Health

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