Molecular properties of the ATP synthetase from Escherichiacoli

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Abstract

The molecular weight, radius of gyration and volume of the ATP synthetase from Escherichiacoli were determined by small angle X-ray scattering in solution. A weight average molecular weight of 448,000 ± 23,000 for the F1·F0-complex was determined by small angle X-ray and laser light scattering at pH 7.8 at 4°C and a molecular weight of 930,000 ± 13,500 was found at 20°C. The maximum cord length of the F1·F0-complex at 4°C was determined to 17.5 ± 1.0 nm and 35.0 ± 2.0 nm at 20°C for the dimer, indicating a linear arrangement of the two F1·F0 monomers within the dimer. A possible hydrodynamic description of the F1·F0 monomer is that of a spherical cone with dimensions of H = 17.0 nm in height, a larger spherical radius of R1 = 8.4 nm and a smaller one of R2 = 5.9 nm, which match the experimental scattering curve satisfactorily.

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