Peripheral proteins of human erythrocytes

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Abstract

Water soluble, nonglycosylated proteins have been extracted from human erythrocyte membranes by two different methods and characterized immunochemically and by PAGE. The spectrin peripheral protein complex (PAGE bands 1 + 2) has been equated with two antigens of intermediate mobility in immunoelectrophoretic analysis of crude spectrin developed with antiserum to bands 1 + 2 purified by elution from gels. Nonspectrin proteins, including catalase, remain in close association with isolated membranes, and display solubility properties similar to those of spectrin. Along with spectrin, they may also function in the intact cell as peripheral proteins.

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