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Transient expression of simple epithelial keratins by mesenchymal cells of regenerating newt limb

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Abstract

Structural proteins of the intermediate filament family are an early indicator of differentiation before organogenesis becomes apparent. Keratin intermediate filaments are characteristically expressed only by epithelial and not by mesenchymal cells. Here we show, using monoclonal antibodies, a transient expression of the keratin pair 8 and 18 in a population of mesenchymal cells in the regenerating newt limb, specifically in the undifferentiated progenitor cells (blastemal cells) which give rise to the new tissues. These keratins are also expressed in cultured limb cells that can differentiate into muscle. In contrast no reactivity with anti-keratin 8 and 18 antibodies was observed in the newt limb bud at an early stage of development, indicating a molecular difference between the developing and regenerating limb. The molecular weights of the newt proteins detected by these antibodies are very similar to those of human keratins 8 and 18, further supporting the immunocytochemical evidence that the newt homologs of these keratins are expressed in blastemal cells. This is the first demonstration of keratin expression in mesenchymal progenitor cells in an adult animal.

References (51)

  • M. Osborn et al.

    Intermediate filaments proteins: A multigene family distinguishing major cell lineages

    Trends Biochem. Sci

    (1986)
  • F. Ramaekers et al.

    Tissue distribution of keratin 7 as monitored by a monoclonal antibody

    Exp. Cell Res

    (1987)
  • F. Ramaekers et al.

    Cytokeratin filament expression during in vitro teratocarcinoma cell differentiation as detected by a monoclonal antibody

    Cell Biol. Int. Rep

    (1984)
  • A. Semat et al.

    Mesenchymal-epithelial conversions induced by 5-azacytidine: Appearance of cytokeratin Endo-A messenger RNA

    Differentiation

    (1986)
  • A. Semat et al.

    Sequence analysis of murine cytokeratin endo A(n0 8) cDNA

    Evidence for mRNA species initiated upstream of the normal 5′ end in PCC4 cells

    Differentiation

    (1988)
  • C.S. Thornton

    Amphibian limb regeneration

    Adv. Morphog

    (1968)
  • G.N.P. Van Muijen et al.

    Differentiation related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn and adult epidermis

    Exp. Cell Res

    (1987)
  • J. Bartek et al.

    A class of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins

    J. Cell Sci

    (1985)
  • J.L. Broers et al.

    Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: A biochemical and immunological analysis using a panel of monoclonal and polyclonal antibodies

    J. Cell Sci

    (1986)
  • J.A. Cameron et al.

    Evidence that reserve cells are a source of regenerated adult newt muscle in vitro

    Nature (London)

    (1986)
  • J.C. Chisholm et al.

    Cytokeratin filament assembly in the preimplantation mouse embryo

    Development

    (1987)
  • D.M. Fekete et al.

    A monoclonal antibody detects a difference in the cellular composition of developing and regenerating limbs of newts

    Development

    (1987)
  • D.M. Fekete et al.

    Evidence that the nerve controls molecular identity of progenitor cells for limb regeneration

    Development

    (1988)
  • P. Ferretti et al.

    A regeneration-associated cytoskeletal antigen is expressed in cultured newt cells

  • P. Ferretti et al.

    Culture of newt cells from different tissues and their expression of a regeneration-associated antigen

    J. Exp. Zool

    (1988)
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