Elsevier

Experimental Cell Research

Volume 123, Issue 2, 15 October 1979, Pages 333-343
Experimental Cell Research

Membrane receptor mobility changes by sendai virus☆

https://doi.org/10.1016/0014-4827(79)90475-0Get rights and content

Abstract

Treatment with Sendai virus does affect the lateral mobility of cell surface components on cultured human cells (KB) and cultured mouse cells (3T3). Diffusion coefficients and mobile fractions of a fluorescent lipid analog, tetramethyl rhodamine-succinyl concanavalin A (ConA) and tetramethyl rhodamine-anti human β2 microglobulin antibodies on the cell surface were measured by fluorescence photobleaching recovery (FPR). Diffusion coefficients of receptors for ConA and for antibodies to β2 microglobulin (presumably histocompatibility antigens) were increased 2- to 3-fold by exposure to the ultraviolet inactivated virus at virus doses from 250 to 2 500 HAU/ml, regardless of whether the particular cells measured had been fused. No mobility enhancement was induced with trypsinized Sendai virus, nor by influenza virus, indicating a probable requirement for the Sendai virus F protein. Virus treatment reduced the diffusion coefficient of a lipid analog in 3T3 cells. The results are compatible with the hypothesis that the Sendai virus disrupts a mobility restraint system, perhaps cytoskeleton-membrane connections.

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    ☆

    This research was supported by grants from the NIH to Cornell University (CA 14454), to E. L. E. (GM-21661) and from NSF to WWW (PCM76-83068).

    2

    T. M. was a recipient of an International Cancer Research Technology Transfer Grant.

    1

    C. A. E. was a recipient of an NIH post-doctoral fellowship (F32-CA05842-02).

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