Original articleDeoxyribonucleoside triphosphate metabolism and the mammalian cell cycle: Effects of hydroxyurea on mutant and wild-type mouse S49 T-lymphoma cells☆
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Cited by (21)
Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication
2017, Molecular CellCitation Excerpt :The differences observed between purine and pyrimidine pools are not fully understood. It has been suggested that pyrimidine levels persist due to compensatory activities of the pyrimidine salvage pathways or as a result of the deoxyuridine present in culture medium, which cells could uptake and phosphorylate, therefore contributing to pyrimidine levels, particularly dTTP (Eriksson et al., 1987; Bianchi et al., 1986). Interestingly, the pyrimidine salvage pathway has been reported to be more efficient than the purine pathway, suggesting an explanation for why purine levels are more sensitive to hydroxyurea (HU) and potentially hypoxia (Reichard, 1988).
Hydroxyurea Arrests DNA Replication by a Mechanism that Preserves Basal dNTP Pools
2004, Journal of Biological ChemistryCitation Excerpt :If dNTP levels were artificially raised by incubating yeast dbf4 mutants at the nonpermissive temperature for S phase entry, HU did not block replication when the cells were subsequently switched to the permissive temperature (Fig. 2). These results are consistent with earlier mammalian cell studies, which showed that exogenous deoxyribonucleosides partially or totally reversed the effect of HU on replication (20, 22, 35–37). The reversal data indicate that RNR activity per se is not needed for DNA chain elongation.
Determination of deoxyribonucleoside triphosphate pool sizes in ribonucleotide reductase cDNA transfected human KB cells
1998, Biochemical PharmacologyEnzyme Organization in DNA Precursor Biosynthesis
1993, Progress in Nucleic Acid Research and Molecular Biology
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This work was supported by grants from the Swedish Medical Research Council, the Swedish Cancer Society, the Medical Faculty of the Karolinska Institute and the Cancer Society of Stockholm.