Differential expression and modulation of Thy-1 on myoblast clones

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Abstract

In the preceding paper [J. S. Schweitzer, M. A. Dichter, and S. J. Kaufman, 1987, Exp. Cell Res.172, 1] we demonstrated that expression of Thy-1 antigen by rat skeletal muscle myoblasts in culture is modulated by fibroblasts. Growth of myoblasts with myofibroblasts, or in medium in which fibroblasts have been grown, causes a sharp reduction in the percentage of myoblasts that express Thy-1 antigen on their membrane. In this paper we demonstrate that there are two populations of myoblasts that can be distinguished by their capacity to respond to the Thy-1-modulating factor (TMF). The majority of Thy-1-positive or -negative myoblasts grown at clonal densities are responsive to TMF. A second myoblast clonal type is Thy-1 positive and fuses to form contractile myotubes, but is insensitive to TMF and has a more fibroblast-like morphology. These clonal phenotypes are stable upon passage in vitro and may represent distinct subsets of the myogenic lineage.

References (27)

  • P.H. Bonner et al.

    Dev. Biol

    (1974)
  • S.D. Hauschka

    Dev. Biol

    (1974)
  • J. Seed et al.

    Dev. Biol

    (1984)
  • R. Rutz et al.

    Dev. Biol

    (1982)
  • P.H. Bonner

    Dev. Biol

    (1975)
  • P.H. Bonner

    Dev. Biol

    (1978)
  • P.H. Bonner

    Dev. Biol

    (1980)
  • P.H. Bonner et al.

    Dev. Biol

    (1982)
  • J.S. Schweitzer et al.

    Exp. Cell Res

    (1987)
  • N.K. White et al.

    Exp. Cell Res

    (1971)
  • N.K. White et al.

    Dev. Biol

    (1975)
  • B.H. Lipton

    Dev. Biol

    (1977)
  • U. Kuhl et al.

    Dev. Biol

    (1982)
  • Cited by (0)

    This work was supported by National Research Service Award 2T 32 GM07753-07 from the NIGMS to J.S.S. and NIGMS Grant GM28842 to S.J.K.

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