Induction of L-alanine:4,5-dioxovalerate transaminase by phenylhydrazine is associated with depletion of heme level

https://doi.org/10.1016/S0006-291X(86)80559-9Get rights and content

Summary

The hemin regulation of L-alanine:4,5-dioxovalerate transaminase, the enzyme proposed for an alternate route of δ-aminolevulinic acid (ALA) biosynthesis in mammalian system was studied in different conditions: phenylhydrazine induced anemia, polycythemia by erythropoietin to anemic rats, treatment with cobalt chloride, a porphyrogenic drug. The activity of L- alanine:4,5-dioxovalerate transaminase in liver and kidney is stimulated in phenylhydrazine, whereas, erythropoietin injection to anemic rats prevents such stimulation. Further treatment with cobalt chloride to erythropoietin treated anemic rats stimulates the enzyme activity. Actinomycin D, however, inhibits the stimulation of L-alanine:4,5-dioxovalerate transaminase by phenylhydrazine suggesting that induction is at the level of transcription. Induced level of this enzyme in anemic condition was estimated quantitatively by radial immunodiffusion using antibody raised against L-alanine:4,5-dioxovalerate transaminase. Moreover, our studies reveal that stimulation of L-alanine:4,5-dioxovalerate transaminase in anemic condition is dependent on depletion of heme level. The regulatory role of intracellular heme pool on the induction of this enzyme suggests its physiological importance in heme biosynthesis.

References (19)

  • VarticovskiL. et al.

    J. Biol. Chem.

    (1980)
  • SinghN.K. et al.

    Biochem. Biophys. Acta

    (1985)
  • OkunoE. et al.

    Biochem. Biophys. Res. Commun.

    (1983)
  • GranickS. et al.
  • ShankerJ. et al.

    FEBS Letters

    (1985)
  • BeruN. et al.

    J. Biol. Chem.

    (1985)
  • ChaudhuriT. et al.

    Biochem. Med.

    (1980)
  • TomaroM.L. et al.

    Biochem. Biophys. Acta

    (1981)
  • ManciniG. et al.

    Immunobiochemistry

    (1965)
There are more references available in the full text version of this article.

Cited by (9)

  • Chicken eggshell porphyrins and the glyoxalase pathway: Its possible physiological role

    1993, Comparative Biochemistry and Physiology -- Part B: Biochemistry and
  • Nonenzymatic synthesis of ∂-aminolevulinate (ALA) by cobalt (CO<sup>++</sup>)

    1987, Biochemical and Biophysical Research Communications
View all citing articles on Scopus
View full text