Identification of a disulfide-bonded 70 Kd Type X procollagen in embryonic chick sternum cartilage

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Summary

Chondrocytes from the presumptive calcification region of 20 day old embryonic chick sternum were found to synthesize a 70 Kd Type X procollagen precursor in addition to the previously described 59 Kd Type X collagen molecules. The 70 Kd molecules exhibited an additional cyanogen bromide peptide, contained a disulfide-bonded domain, and were converted into the 59 Kd moieties during pulse-chase experiments. The conversion of the 70 Kd to the 59 Kd Type X collagen was prevented upon microtubular transport inhibition with colchicine and resulted in tissue accumulation of the 70 Kd Type X procollagen.

References (14)

  • SchmidTM et al.

    J Biol Chem

    (1982)
  • SchmidTM et al.

    J Biol Chem

    (1982)
  • RemingtonMC et al.

    Collagen Relat Res

    (1983)
  • SchmidTM et al.

    J Biol Chem

    (1983)
  • CastagnolaP et al.

    Biochem Biophys Res Commun

    (1986)
  • YoshimuraM et al.

    J Biol Chem

    (1981)
  • ReginatoAM et al.

    J Biol Chem

    (1986)
There are more references available in the full text version of this article.

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