Platelet activating factor and leukotriene B4 induce hyperpolarisation of human endothelial cells but depolarization of neutrophils

https://doi.org/10.1016/S0006-291X(88)81167-7Get rights and content

We studied one expression of cell activation in neutrophils (PMN) and endothelial cells (EC), membrane potential changes [assessed by the fluorescent dye, di-C-O5(3)]. Human neutrophils responded with depolarization after exposure to fMLP, LTB4, A23187, PAF and PMA. In contrast, only PAF and LTB4 induced membrane potential changes in human umbilical vein EC, which responded with increased fluorescence, possibly indicating membrane hyperpolarization. These discordant responses may reflect processes of significance for interactions between EC and PMN.

References (20)

  • HarlanJ.M.

    Blood

    (1985)
  • HarlanJ.M. et al.

    Blood

    (1985)
  • Bevilacqua, M.P., Pober, J.S., Mendrick, D.L., Cotran, R.S. and Gimbrone, M.A.Jr. (1987) 84(24),...
  • GallinJ.I.

    J Clin Invest

    (1980)
  • GallinJ.I. et al.

    J Clin Invest

    (1975)
  • BussolinoF. et al.

    J Immunol

    (1987)
  • ZimmermanG.A. et al.

    J Clin Invest

    (1985)
  • McIntyreT.M. et al.
  • SeligmannB.E. et al.

    J Clin Invest

    (1980)
  • SeligmannB.E. et al.

    J Membrane Biol

    (1980)
There are more references available in the full text version of this article.

Cited by (22)

  • Effects of Platelet-Activating Factor on Brain Microvascular Endothelial Cells

    2018, Neuroscience
    Citation Excerpt :

    We also identified that PAF depolarized RBMVEC loaded with a slow response voltage-sensitive fluorescent dye, DiBAC4(3); the response was significantly diminished by antagonism of PAF receptor or by inhibition of Ca2+ influx. Similarly, earlier studies reported that PAF depolarized human or rabbit neutrophils (Naccache et al., 1986; Lerner et al., 1988) human platelets (Avdonin et al., 1991), bovine oviductal cells (Tiemann et al., 1999) or rat stomach fundus (Kamata et al., 1993a). Depolarization of vascular endothelial cells was associated with NO production and increased cellular stiffness via reorganization of cortical actin cytoskeleton (Callies et al., 2011).

  • Platelet-activating factor (PAF): Implications for coronary heart and vascular diseases

    1994, Prostaglandins, Leukotrienes and Essential Fatty Acids
  • The role of platelet-activating factor in inflammation

    1990, Clinical Immunology and Immunopathology
  • Leukotriene B4 induced hyperadhesiveness of endothelial cells for neutrophils

    1990, Biochemical and Biophysical Research Communications
View all citing articles on Scopus
View full text