Characterization of receptor for granulocyte colony-stimulating factor on human circulating neutrophils

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Summary

We have made a mutein of human G-CSF with more stable, and potent biological activity. Using 125I-labeled mutein human G-CSF, high affinity binding sites were identified on human circulating neutrophils. Receptor number per cell was 560 with a Kd of 250 pM. The human G-CSF receptor was identified as a single subunit protein of Mr approximately 150,000.

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