Immunological evidence for the localization of sialoglycosphingolipids at the at the cell surface of sea urchin spermatozoa

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Abstract

The localization of sialoglycosphingolipids in the plasma membrane of sea urchin spermatozoa was studied by employing immunological methods including immunolysis of liposomal model membranes.

The antibodies produced against these complex lipids were found to agglutinate various sea urchin spermatozoa differently. Both species differences and species similiraties in the agglutination were found in spermatozoa of the echinoderm, the sea urchin and the starfish. The agglutination of the sea urchin spermatozoa was inhibited specifically by certain carbohydrates.

Only a limited number of molecular species of sialoglycosphingolipid were localized at the surface of the plasma membrane of sea urchin spermatozoa cells. Moreover, topographical differences were found in the localization of the sialoglycosphinolipids in the cell surface of spermatozoa. at the cell surface of spermatozoa.

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This is Part VII of the series Biochemistry of Lipids of Sea Urchin Gametes and Embryos.

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