Studies on intestinal hydrolysis of peptides I. l-Glutaminyl-l-proline dipeptide hydrolase activity in the human small intestine

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Abstract

The activities of l-glutaminyl-l-proline dipeptide hydrolase and glycyl-l-proline dipeptide hydrolase (EC 3.4.3.7) have been studied in the human intestinal mucosa. Both jejunum and ileum demonstrate marked activities, the jejunum being more efficient than the ileum. Characterization studies have been performed on partially purified enzymes obtained by gel filtration. l-Glutaminyl-l-proline dipeptide hydrolase and glycyl-l-proline dipeptide hydrolase are different with respect to pH and ion dependence. The two enzyme activities can be separated with heat treatment in the presence of Mn2+.

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    Present address: Children's Hospital Medical Center, 300 Longwood Avenue, Boston, Mass. 02115, U.S.A.

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