Rapid communication
Effect of androgen and thyroid hormones on renin-1 messenger ribonucleic acid levels in mouse submandibular gland

https://doi.org/10.1016/0303-7207(88)90070-6Get rights and content

Abstract

The synthesis of renin and other biologically active polypeptides in the granular convoluted tubule cells of the mouse submandibular gland (SMG) is regulated by androgen and thyroid hormones. In this study genetically hypothyroid (hyt/hyt) mice carrying a single renin structural gene (Ren-1) were used to investigate the mechanism of hormonal action in mouse SMG. Treatment of female mice with 5α-dihydrotestosterone (DHT) and/or thyroxine (T4) enhanced renin-1 activity and increased renin-1 mRNA, determined by Northern analysis. Compared to euthyroid (hyt/ +) littermates, hyt/hyt mice had lower basal levels of renin-1 mRNA and a blunted response to either hormone alone. DHT and T4 acted synergistically to increase renin-1 activity and renin-1 mRNA in the SMG of hyt/hyt females. Furthermore, levels of renin-1 activity and renin-1 mRNA varied concordantly in the SMG of these animals. These data indicate that androgen and thyroid hormones influence levels of renin-1 in mouse SMG primarily by regulating the amount of renin-1 mRNA available for translation.

References (25)

  • O.H. Lowry et al.

    J. Biol. Chem.

    (1951)
  • N. Piccini et al.

    Cell

    (1982)
  • F. Soubrier et al.

    Gene

    (1986)
  • C.M. Wilson et al.

    Life Sci.

    (1982)
  • T. Barka

    J. Histochem. Cytochem.

    (1980)
  • W.G. Beamer et al.

    Science

    (1981)
  • J. Bing et al.

    Acta Pathol. Microbiol

    Immunol. Scand. Sect. A Pathol.

    (1971)
  • D.F. Catanzaro et al.

    Endocrinology

    (1985)
  • D.P. Dickinson et al.

    Genetics

    (1984)
  • L.J. Field et al.
  • L.J. Field et al.

    Hypertension

    (1984)
  • E.W. Gresik et al.

    Cell Tissue Res.

    (1975)
  • Cited by (0)

    Present address: Ludwig Institute for Cancer Research, 687 Pine Ave. West, Montreal P.Q. H3A 1A1 Canada.

    View full text