Induction and repression of enzymes involved in exogenous purine compound utilization in Bacillus cereus

https://doi.org/10.1016/0304-4165(81)90127-6Get rights and content

Abstract

5′-Nucleotidase, adenosine phosphorylase, adenosine deaminase and purine nucleoside phosphorylase, four enzymes involved in the utilization of exogenous purine compounds in Bacillus cereus, were measured in extracts of this organism grown in different conditions. It was found that adenosine deaminase is inducible by addition of adenine derivatives to the growth medium, and purine nucleoside phosphorylase by metabolizable purine and pyrimidine ribonucleosides. Adenosine deaminase is repressed by inosine, while both enzymes are repressed by glucose. Evidence is presented at during growth of B. cereus in the presence of AMP, the concerted action of 5′-nucleotidase and adenosine phosphorylase, two constitutive enzymes, leads to formation of adenine, and thereby to induction of adenosine deaminase. The ionsine formed would then cause induction of the purine nucleoside phosphorylase and repression of the deaminase. Taken together with our previous findings showing that purine nucleoside phosphorylase of B cereus acts as a translocase of the ribose moiety of ionsine inside the cell (Mura, U., Sgarrella, F. and Ipata, P.L. (1978) J. Biol. Chem. 253, 7905–7909), our results provide a clear picture of the molecular events leading to the utilization of the sugar moiety of exogenous AMP, adenosine and inosine as an energy source.

References (19)

  • U. Mura et al.

    J. Biol. Chem.

    (1978)
  • S. Senesi et al.

    FEBS Lett.

    (1976)
  • R. Gardner et al.

    J. Biol. Chem.

    (1967)
  • P.L. Ipata

    Anal. Biochem.

    (1967)
  • H.M. Kalckar

    J. Biol. Chem.

    (1947)
  • H.M. Kalckar

    J. Biol. Chem.

    (1947)
  • R.P. Miech et al.

    Biochem. Pharmacol.

    (1975)
  • U. Mura et al.

    Anal. Biochem.

    (1979)
  • J. Hochstadt-Ozer et al.

    J. Biol. Chem.

    (1971)
There are more references available in the full text version of this article.

Cited by (0)

View full text