Elsevier

Gene

Volume 32, Issues 1–2, December 1984, Pages 217-224
Gene

A new selective phage cloning vector, λ2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI

https://doi.org/10.1016/0378-1119(84)90049-0Get rights and content

Abstract

An improved bacteriophage λ cloning vector, λ2001, has been constructed. The phage includes a 34-bp Polylinker oligonucleotide which introduces cleavage sites for XbaI, SstI, XhoI, EcoRI, HindIII and BamHI, and can accommodate 10-kb to 23-kb fragments. Inserts that destroy the BamHI or XhoI cloning sites may be recovered by excision at flanking sites in the Polylinker sequence. Insertion of foreign DNA into λ2001 generates phage with a Spi phenotype. The recombinant phage are able to grow on P2 lysogens but the parental vector phages are not. In the course of this work, the Polylinker sequence was also introduced into M13mp8. This produced a new vector, M13mp12, with cloning sites for EcoRI, SmaI, XbaI, SstI, XhoI, BamHI, and HindIII.

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    Present address: CNRS, Laboratoire de Génétique Moléculaire des Eucaryotes, Faculté de Médecine, 11 Rue Humann, 67085 Strasbourg Cedex (France).

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