Effects of the umuDC, mucAB, and samAB operons on the mutational specificity of chemical mutagenesis in Escherichia coli: II. Base substitution mutagenesis

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Abstract

Mutational spectra induced by different classes of chemical mutagens including two ultraviolet-mimetic mutagens, an alkylating agent, intercalators, a crosslinking agent, and base analogs were characterized by means of a set of mutant lacZ genes in E. coli. These strains can be used to detect each of two types of transition and four types of transversion, simply by measuring the number of Lac+ revertant colonies. 4-Nitroquinoline 1-oxide induced G·C → A·T, G·C → C·G, or G·C → T·A changes almost equally, whereas furylfuramide and mitomycin C induced only G·C → A·T transitions and G·C → T·A transversions, respectively. No base substitutional mutations were detected by the treatment with 9-aminoacridine. A weak stimulation of G·C → A·T transitions by ICR-191 was observed. Both the G·C → A·T and A·T → G·C transitions were induced by N-methylN′-nitro-N-nitrosoguanidine and N4-aminocytidine. 5-Azacytidine was a specific inducer of G·C → C·G transversions.

In addition, a comparative study of mutational specificity was performed in the strains bearing either the umuDC, mucAB, or the samAB operon on a multicopy plasmid. Regardless of the kind of mutagen, G·T → T·A transversions were greatly potentiated by the introduction of plasmids in the order of pGW1700 (mucAB) >pSE117 (umuDC) ≧ pYG8011 (samAB). Besides G·C → T·A transversions, the introduction of pGW1700, but not pSE117 and pYG8011, enhanced the mutations of A·T → C·G and A·T → T·A transversions. The mucAB plasmid also enhanced the G·C → A·T transitions and G·C → C·G transversions induced by some mutagens.

References (45)

  • B. Bailleul et al.

    Adducts from the reaction of O,O′-diacetyl or O-acetyl derivatives of the carcinogen 4-hydroxy-aminoquinoline 1-oxide with purine nucleosides

    Cancer Res.

    (1981)
  • M. Blanco et al.

    Different efficiency of UmuDC and MucAB proteins in UV light induced mutagenesis in Escherichia coli

    Mol. Gen. Genet.

    (1986)
  • C. Coulondre et al.

    Genetic studies of the lac repressor, IV. Mutagenic specificity in the lacI gene of Escherichia coli

    J. Mol. Biol.

    (1977)
  • C.G. Cupples et al.

    A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions

  • C.G. Cupples et al.

    A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations

    Genetics

    (1990)
  • P. Daubersies et al.

    Mutation spectra of the two guanine adducts of the carcinogen 4-nitroquinoline 1-oxide in Escherichia coli. Influence of neighbouring base sequence on mutagenesis

    Carcinogen

    (1992)
  • L.R. Ferguson et al.

    Comparative studies of mutagenic, DNA binding and antileukemic properties of 9-anilinoacridine derivatives and related compounds

    Chem.-Biol. Interact.

    (1983)
  • P.L. Foster et al.

    Induction of transverse mutations in Escherichia coli by N-methyl-N′-nitro-N-nitrosoguanidine is SOS dependent

    J. Bacteriol.

    (1985)
  • R.P.P. Fuchs et al.

    Hot spots of frameshift mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene

    Nature

    (1981)
  • S. Galiegue-Zouitina et al.

    N2-Guanyl and N6adenyl arylation of chicken erythrocytes DNA by the ultimate carcinogen of 4-nitroquinoline 1-oxide

    Cancer Res.

    (1986)
  • J.A. Glazebrook et al.

    Molecular analysis of the UV protection and mutation genes carried by the I incompatibility group plasmid TP110

    J. Bacteriol.

    (1986)
  • T. Kada

    Escherichia coli mutagenicity of furylfuramide

    Jpn. J. Genet.

    (1973)
  • T. Kato et al.

    Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light

    Mol. Gen. Genet.

    (1977)
  • T. Kato et al.

    Effects of the umuc36 mutation on ultraviolet-radiation induced base-change and frameshift mutations in Escherichia coli

    Mutation Res.

    (1981)
  • Y. Kitagawa et al.

    Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli

  • S. Kondo et al.

    Testing and classification of mutagenicity of furylfuramide in Escherichia coli

    Jpn. J. Genet.

    (1973)
  • D.E. Levin et al.

    Classifying mutagens as to their specificity in causing the six possible transitions and transversions: a simple analysis using the Salmonella mutagenicity assay

    Environ. Mutagen.

    (1986)
  • D. Lodwick et al.

    Distribution of sequences homologous to the impCAB operon of TP100 among bacterial plasmids of different incompatibility groups

    Mol. Gen. Genet.

    (1991)
  • D. Lodwick et al.

    DNA sequence analysis of the imp UV protection and mutation operon of the plasmid TP110: identification of a third gene

    Nucleic Acids Res.

    (1990)
  • T. Maniatis et al.
  • L. Marsh et al.

    Cold sensitivity induced by overproduction of UmuDC in Escherichia coli

    J. Bacteriol.

    (1985)
  • T.V. McCarthy et al.

    Inducible repair of O-alkylated DNA pyrimidines in Escherichia coli

    EMBO J.

    (1984)

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