Mutagenic interaction of sodium azide on mutations induced in barley seeds treated with diethyl sulfate or N-methyl-N′-nitrosourea

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Abstract

The effects of sodium azide (NaN3) in combination with diethyl sulfate (dES) or N-methyl-N′-nitrosourea (MNH) on mutation frequency in barley were studied. It was found that sodium azide produced high frequencies of chlorophyll mutations when used alone and has a synergistic effect on mutation yields following MNH treatments. However, the mutation frequency was decreased whe azide was applied following dES treatment of seeds. The mutagenic efficiency of azide was found to be high, possibly because of low “physiological” damage. The synergistic increase in mutation yields by MNH and azide treatment indicates that azide has unusual promise as a mutagen for both practical and research applications.

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Scientific Paper No. 4388. College of Agriculture Research Center, Washington State University, Pullman, Projects 1568 and 1068. Supported in part by the U.S. Atomic Energy Commission Contract AT(45-1)-2221. AEC Paper RLO-2221-T2-14.

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