Journal of Chromatography B: Biomedical Sciences and Applications
Determination of very-long-chain fatty acids in plasma by a simplified gas chromatographic—mass spectrometric procedure
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2013, Clinical Neurology and NeurosurgeryCitation Excerpt :The slowly progressive course, adrenal insufficiency and symmetric lesions shown in the brain MRI images, AMN phenotype of ALD was suspected. Using a small sample volume from plasma after adequate extraction and derivatization, methyl esters of VLCFA were separated, identified and quantified by gas chromatography–mass spectrometry [3]. The results showed marked elevation in the C24:0/C22:0 ratio at 1.4434 (normal range: 0.231–0.892) and the C26:0/C22:0 at 0.1414 (normal range: 0.0054–0.0235).
Plasma fatty acids metabolic profiling analysis of coronary heart disease based on GC-MS and pattern recognition
2009, Journal of Pharmaceutical and Biomedical AnalysisA simple and sensitive liquid chromatographic method for the analysis of free docosanoic, tetracosanoic and hexacosanoic acids in human plasma as fluorescent derivatives
2008, Analytica Chimica ActaCitation Excerpt :The analytical results of the free VLCFA in ALD patients are compatible with that reported [9]. The proposed method is highly sensitive with the limit of detection (5.0 nM; LOD) comparable to that of GC–MS methods having LOD ranged from 2.5 to 50 nM [11–13]. A simple and sensitive method with readily available apparatus was developed for the analysis of VLCFA in human plasma by fluorimetric derivatization and isocratic LC.
Chromatography of very long-chain fatty acids from animal and plant kingdoms
2002, Analytica Chimica ActaAnalysis of fatty acids in plasma lipoproteins by gas chromatography-flame ionization detection: Quantitative aspects
2002, Analytica Chimica ActaCitation Excerpt :Columns are 50–100 m long, however, for satisfactory resolution of cis–trans isomers 18:1 previous separation on silver nitrate thin-layer chromatography (TLC) is often necessary [17]. Combination of GC with mass spectrometric (MS) detector is the best configuration for analysis of samples containing unusual FA [18–20], however, the high cost of such equipment is the reason why low-cost flame ionization detector (FID) is still widely used. Thermal conductivity detectors (TCD) were also used for FAME analysis in early 1970s [21,22], with results comparable to FID, at present they are used mostly for analyses of gaseous mixtures.
Gas chromatographic and mass spectrometric methods for the characterisation of long-chain fatty acids: Application to wool wax extracts
2002, Analytica Chimica ActaCitation Excerpt :Some recent papers describe rapid and precise methods for the determination of very long-chain fatty acids (VLCFAs, acids with the carbon atom range C22–C28) in human serum (small sample volume) for clinical diagnosis [47–49]. For the determination of VLCFA (as FAME) at clinically significant level of precision (around 50 nmol/l in plasma, equivalent to 20 ng/ml), a GC/MS method in selected ion monitoring (SIM) has been developed [47]. The method is sensitive enough for clinical diagnosis but is time consuming because of the derivative preparation step.