A comparative study of protein immobilization techniques for optical immunosensors
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Ellipsometric biosensors
2023, Fundamentals of Sensor Technology: Principles and Novel DesignsProtein immobilization onto various surfaces using a polymer-bound isocyanate
2015, Applied Surface ScienceCitation Excerpt :Therefore, the immobilization time was fixed at 240 min for subsequent experiments. The immobilization time (e.g., 240 min) falls well within the values (2 h to overnight) of conventional silane-based protein immobilization methods [23]. The protein immobilization efficiency of the newly developed method was compared with the immobilization efficiency of the conventional silane-based method.
Optimization and characterization of biomolecule immobilization on silicon substrates using (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde linker
2014, Applied Surface ScienceCitation Excerpt :They also used mixed silane composition (i.e. APTES and methyl-triethoxysilane (MTES)) for covalent immobilization of antibody to reduce the number of APTES molecules on the surface [9]. Ahluwalia et al. [26] studied a number of antibody immobilization techniques using APTES silane for applications to optical immunosensors. They focused on the covalent binding and physical adsorption of proteins to improve the density and uniformity in orientation of antibodies on the substrate.
Detection of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) by displacement of antibodies
2011, Sensors and Actuators, B: ChemicalCitation Excerpt :In immunoassays, antibodies are normally bound to the surface by methods that give a random immobilisation and only few of the antibodies are available for binding of the target molecules. If low concentrations of target molecules are to be detected the antibodies have to be site-directly immobilised on the surface and the non-specific binding has to be low [3,4]. If these conditions are fulfilled it may still, however, be difficult to detect low concentrations of small-sized molecules.
Characterization of silanization and antibody immobilization on spin-on glass (SOG) surface
2009, Applied Surface ScienceCitation Excerpt :The glutaraldehyde treatment was found to increase the thickness by 0.498 nm. This resembles results reported previously [10]. The antibody layer thickness obtained was 4.78 nm, which is close to the expected range of 5–10 nm.