Conventional and laser induced fluorescence detection of glucuronic acid conjugates after derivatization and liquid chromatographic separation

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Abstract

Pre-chromatographic derivatisation of the carboxylic acid function of glucuronic acid conjugates is a suitable method for the selective and ultra-sensitive analysis of these compounds in urine and plasma samples. This goal is achieved by applying an indirect derivatisation procedure and laser induced fluorescence detection with a homemade detection system equipped with a continuous-wave argon-ion laser. The minimum detectable amounts for the analytes, after derivatisation, are about 3 amol using the fluorescein fluorophore. In comparison with conventional induced fluorescence detection a gain in sensitivity of over four orders of magnitude is obtained.

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Presented at the “International Symposium on Pharmaceutical and Biomedical Analysis”, September 1987, Barcelona, Spain.

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