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The X-ray crystal structure of phosphomannose isomerase from Candida albicans at 1.7 Å resolution

Nature Structural Biology volume 3, pages 470–479 (1996)Cite this article

Abstract

Phosphomannose isomerase (PMI) catalyses the reversible isomerization of fructose-6-phosphate (F6P) and mannose-6-phosphate (M6P). Absence of PMI activity in yeasts causes cell lysis and thus the enzyme is a potential target for inhibition and may be a route to antifungal drugs. The 1.7 ˚ crystal structure of PMI from Candida albicans shows that the enzyme has three distinct domains. The active site lies in the central domain, contains a single essential zinc atom, and forms a deep, open cavity of suitable dimensions to contain M6P or F6P. The central domain is flanked by a helical domain on one side and a jelly-roll like domain on the other.

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Authors and Affiliations

  1. Glaxo Wellcome Research and Development Department of Biomolecular Structure, Glaxo Wellcome Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK

    Anne Cleasby, Alan Wonacott & Tadeusz Skarzynski

  2. Department of Chemistry, University of York, Heslington, York, Y01 5DD, UK

    Roderick E. Hubbard & Gideon J. Davies

  3. Glaxo Institute of Molecular Biology, 1228 Plan-les-Ouates, Geneva, Switzerland

    Amanda E. I. Proudfoot, Alain R. Bernard, Mark A. Payton & Timothy N.C. Wells

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Cleasby, A., Wonacott, A., Skarzynski, T. et al. The X-ray crystal structure of phosphomannose isomerase from Candida albicans at 1.7 Å resolution. Nat Struct Mol Biol 3, 470–479 (1996). https://doi.org/10.1038/nsb0596-470

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