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Culture pH Affects Expression Rates and Glycosylation of Recombinant Mouse Placental Lactogen Proteins by Chinese Hamster Ovary (CHO) Cells

Bio/Technology volume 11, pages 720–724 (1993)Cite this article

Abstract

Glycosylation patterns and specific expression rates of the recombinant protein mouse placental lactogen-I (mPL-I) by Chinese hamster ovary (CHO) cells varied significantly over the extracellular pH (pHe) range of 6.1 to 8.7. The maximum specific mPL-I expression rates occurred between pHe 7.6 and 8.0. The pHe effect on protein expression was confirmed using a different CHO cell expressing the unglycosylated recombinant protein mouse placental lactogen-II (mPL-II). Decreases in the extent of glycosylation of mPL-I were observed at low (below 6.9) and high (above 8.2) pHe values. The pHe dependent variations in mPL-I accumulation in the supernatant as well as in glycosylation patterns were not the result of enzymatic degradation in the culture medium.

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Author notes

  1. Michael C. Borys

    Present address: Abbott Laboratories, North Chicago, IL, 60064

  2. Eleftherios T. Papoutsakis: Corresponding author.

Authors and Affiliations

  1. Department of Chemical Engineering, Northwestern University, Evanston, IL, 60208

    Michael C. Borys & Eleftherios T. Papoutsakis

  2. Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL, 60208

    Daniel I.H. Linzer

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Borys, M., Linzer, D. & Papoutsakis, E. Culture pH Affects Expression Rates and Glycosylation of Recombinant Mouse Placental Lactogen Proteins by Chinese Hamster Ovary (CHO) Cells. Nat Biotechnol 11, 720–724 (1993). https://doi.org/10.1038/nbt0693-720

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