Abstract
IT was determined that plain agar plates infused with spores of Bacillus subtilis could be used to determine the bacteristatic effect of the venom of Hymenoptera. Peptone–glucose nutrient paper disks wetted with bee venom and placed on the agar surface produced uniform and measurable zones of growth inhibition which varied directly with the concentration of the venom. A reverse phase, bioassay method of standardizing solutions of Hymenoptera venom is proposed and a unit of venom is defined.
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References
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BENTON, A., MORSE, R. & KOSIKOWSKI, F. Bioassay and Standardization of Venom of the Honey Bee. Nature 198, 295–296 (1963). https://doi.org/10.1038/198295b0
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DOI: https://doi.org/10.1038/198295b0
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