Abstract
CONIDIA of the mould Aspergillus nidulans were unexpectedly found to require a small amount of biotin (or larger amounts of desthiobiotin) for initial growth, when plated on to a minimal medium of Czapek–Dox, at a density of no more than 103 per dish. This fact seems to be in contradiction to what was previously considered to be their basic requirement. Biotin must be added to the minimal medium, for a total germination of wild-type conidia, to the extent of 10−6gm./ml. This corresponds to about 1/100–1/10 of the amount required by the biotinless mutant bi-l.
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STRIGINI, P., MORPURGO, G. Biotin Requirement and Carbon and Sulphur Sources in Aspergillus and Neurospora . Nature 190, 557 (1961). https://doi.org/10.1038/190557a0
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DOI: https://doi.org/10.1038/190557a0
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