Abstract
NUMEROUS papers have been published concerning the identification and determination of amino-acids by the dinitrophenylation method introduced by Sanger1. We are not aware, however, of any indications in the literature about the occurrence of polymerization reactions during dinitrophenylation. In an attempt to work out a procedure for the quantitative determination of minute amounts of ɛ-amino-caproic acid and its linear oligomers, we have found that such polymerization reactions do occur. Similar results have been found during dinitrophenylation of other ω-amino-acids and of glycine and β-alanine. Since these side-reactions may lead to complications in investigations on the separation and determination of dinitrophenyl amino-acids in general, it seems useful to communicate these facts briefly.
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References
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HEIKENS, D., HERMANS, P. & VAN VELDEN, P. Polymerization Reactions occurring during Dinitrophenylation of α-, β- and ω-Amino-acids with Sanger's Reagent. Nature 174, 1187–1188 (1954). https://doi.org/10.1038/1741187b0
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DOI: https://doi.org/10.1038/1741187b0
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