Identification of Amino-Acids Present in Purified Prothrombin by Circular Paper Chromatography

Abstract

SEEGERS et al. ¹ have very recently made an analysis on the amino-acid content, particularly tyrosine and tryptophan, of purified prothrombin. They have also shown by electrophoresis that their prothrombin preparation contains approximately 90 per cent of the protein in one component¹. Several other workers^2–4 have investigated the nature of the protein in prothrombin. Astrup and Darling⁴ have reported prothrombin to be a protein of globulin character. But the complete analysis of the prothrombin molecule with respect to different amino-acid contents is lacking.