Malformations in Mouse Embryos induced by Trypan Blue

Abstract

EXPERIMENTS in this laboratory, using inbred mice of the CBA strain, have given results similar to those described above by Hamburgh on the Bagg Albino strain. After some preliminary experiments, we used the following procedure. Males were placed with females overnight and the females were examined next morning for copulation plugs. Seven days after the plug, each experimental female was given a single subcutaneous injection of ½ c.c. of 1 per cent aqueous trypan blue; an equal number of females were kept as untreated controls. Embryos were dissected out 1, 2 … 9 days later, that is, at 8½, 9½ … 16½ days embryonic age. Four females were used at each stage in each series; females without implantations were discounted and replaced.